Composite

Part:BBa_K4399012:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-09-27)


The inducible anthocyanin biosynthesis system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal XbaI site found at 8415
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
    Illegal PstI site found at 8599
    Illegal PstI site found at 8770
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal NheI site found at 1244
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
    Illegal PstI site found at 8599
    Illegal PstI site found at 8770
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal BglII site found at 4113
    Illegal BglII site found at 4925
    Illegal BglII site found at 6994
    Illegal BglII site found at 7321
    Illegal BglII site found at 8549
    Illegal BamHI site found at 3547
    Illegal BamHI site found at 3849
    Illegal BamHI site found at 5168
    Illegal BamHI site found at 5237
    Illegal BamHI site found at 8118
    Illegal XhoI site found at 7525
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal XbaI site found at 8415
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
    Illegal PstI site found at 8599
    Illegal PstI site found at 8770
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal XbaI site found at 8415
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
    Illegal PstI site found at 8599
    Illegal PstI site found at 8770
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constitutively expressed XVE (Ba_K4399014) transcription factor could be activated in presence of β-estrogen, then entry cell nucleus, bind to LexA-35S (BBa_K3900024) promoter, thus activate expression of SbMYB75 (BBa_K4399003) and SbDEL (BBa_K4399004). The two transcription factor can switch on endogenous structural genes for anthocyanins biosynthesis. The constitutive GFP (BBa_K4399002) expression box was employed to facilitate screening of transformants.


Source

The sequence is based on BBa_K4399008, BBa_K4399009, BBa_K4399010 and BBa_K4399011.

References